Glucoamylase::green fluorescent protein fusions to monitor protein secretion in Aspergillus niger

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Glucoamylase::green fluorescent protein fusions to monitor protein secretion in Aspergillus niger.

A glucoamylase::green fluorescent protein fusion (GLA::sGFP) was constructed which allows the green fluorescent protein to be used as an in vivo reporter of protein secretion in Aspergillus niger. Two secretory fusions were designed for secretion of GLA::sGFP which employed slightly different lengths of the glucoamylase protein (GLA499 and GLA514). Expression of GLA::sGFP revealed that fluoresc...

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ژورنال

عنوان ژورنال: Microbiology

سال: 2000

ISSN: 1350-0872,1465-2080

DOI: 10.1099/00221287-146-2-415